牡荆苷对脑缺血再灌注损伤小鼠抗氧化酶的影响

开篇：润墨网以专业的文秘视角，为您筛选了一篇牡荆苷对脑缺血再灌注损伤小鼠抗氧化酶的影响范文，如需获取更多写作素材，在线客服老师一对一协助。欢迎您的阅读与分享！

[摘要] 目的 研究牡G苷对脑缺血再灌注损伤小鼠抗氧化酶的作用及机制。 方法 雄性昆明种小鼠70只，按随机数字表法分为正常对照组、假手术组、模型组、牡荆苷组（10、20、40 mg/kg）、尼莫地平组（0.6 mg/kg），采用线栓法制备脑缺血再灌注模型，牡荆苷组在缺血再灌注前连续腹腔给予牡荆苷3 d，缺血再灌注后，每天给药1次，连续给药7 d，检测小鼠神经功能损伤评分，采用试剂盒法检测脑组织、血清中丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、髓过氧化物酶（MPO）的活性。 结果 与假手术组比较，模型组神经功能损伤评分显著升高（P < 0.01）；与模型组比较，牡荆苷组神经功能损伤评分显著降低（P < 0.01）。与假手术组比较，模型组脑组织、血清中MDA含量、MPO活性显著升高，SOD、GSH-Px、CAT活性显著降低，差异均有高度统计学意义（P < 0.01）；与模型组比较，尼莫地平组和牡荆苷组脑组织、血清中MDA含量、MPO活性显著降低，SOD、GSH-Px、CAT活性显著增强，差异有统计学意义（P < 0.01或P < 0.05）。与尼莫地平组比较，10、20 mg/kg牡荆苷组MDA含量和MPO活性显著上升，SOD、GSH-Px、CAT活性显著降低（P < 0.05）；40 mg/kg牡荆苷组与尼莫地平组比较差异无统计学意义（P > 0.05）。 结论 牡荆苷对小鼠脑缺血再灌注损伤的保护作用可能通过抗氧化作用来实现。

[关键词] 牡荆苷；脑缺血再灌注；抗氧化酶

[中图分类号] R743.3 [文献标识码] A [文章编号] 1673-7210（2016）11（c）-0012-05

Effects of vitexin on antioxidant enzymes in cerebral ischemia-reperfusion injury mice

YAN Juan1 ZHENG Maodong1 CUI Yuhuan2 ZHAO Hongxiu1 ZHAO Xiuhua1 BAN Xuxia1

1.Department of Pharmacy， the First Affiliated Hospital of Hebei North University， Hebei Province， Zhangjiakou 075000， China； 2.Department of Geriatrics， the First Affiliated Hospital of Hebei North University， Hebei Province， Zhangjiakou 075000， China

[Abstract] Objective To study the effects and mechanism of vitexin on antioxidant enzymes in cerebral ischemia-reperfusion injury mice. Methods Seventy Kunming male mice were randomly divided into the normal control group， sham group， model group， vitexin group （10， 20， 40 mg/kg） and Nimodipine group （0.6 mg/kg）. Mice were prepared with cereral ischemia-reperfusion injury by suture method. Vitexin was intraperitoneally injected three days before cerebral ischemia， after ischemia-reperfusion， mice were given drugs once daily for seven consecutive days， then neurological deficits were determined. The contents of malondialdehyde （MDA）， the activities of superoxide dismutase （SOD）， catalase （CAT）， glutathione peroxidase （GSH-Px）， and myeloperoxidase （MPO） in brain tissue and serum were determined by the kits methods. Results Compared with sham group， the neurological score was remarkably increased in model group （P < 0.01）； compared with model group， the neurological scores in vitexin group were significantly decreased （P < 0.01）. Compared with sham group， the content of MDA and the activity of MPO in model group were increased， the activities of SOD， CAT， GSH-Px in brain tissue and serum were reduced， the differences were all highly statistically significant （P < 0.01）. Compared with model group， the content of MDA and the activity of MPO of brain tissue and serum in Nimodipine group and vitexin group were decreased， the activities of SOD， CAT， GSH-Px were strengthened， the differences were statistically significant （P < 0.01 or P < 0.05）. Compared with Nimodipine group， the content of MDA and the activity of MPO in 10， 20 mg/kg of vitexin group were increased， the activities of SOD， CAT， GSH-Px were reduced （P < 0.05）， there were no significant differences between 40 mg/kg of vitexin group and Nimodipine group （P > 0.05）. Conclusion Vitexin against cerebral ischemia-reperfusion injury might through its anti-oxidative effect.

[6] 周欣，范国荣，吴玉田.短瓣金莲花总黄酮及指标性成分的体外抗氧化活性研究[J].中药材，2007，30（8）：1000-1002.

[7] 颜娟，郑茂东，崔玉环，等.牡荆苷对小鼠脑缺血再灌注损伤抗氧化应激作用[J].河北北方学院学报：自然科学版，2016，32（2）：19-22.

[8] Himori N，Watanabe H，Akaike N，et al. Cerebral ischemia model with conscious mice. Involvement of NMDA receptor activation and derangement of learning and memory ability [J]. J Pharmacol Methods，1990，23（4）：311-327.

[9] Silveira JP，Seito LN，Eberlin S，et al. Photoprotective and antioxidant effects of Rhubarb：Inhibitory action on tyrosinase and tyrosine kinase activities and TNF-alpha，IL-1alpha and alpha-MSH production in human melanocytes [J]. BMC Complement Altern Med，2013，13（1）：49

[10] Wang TF，Lei Z，Li YX，et al. Oxysophoridine protects against focal cerebral ischemic injury by inhibiting oxidative stress and apoptosis in mice [J]. Neurochem Res，2013，38（11）：2408-2417.

[11] Chao XD，Ma YH，Luo P，et al. Up-regulation of heme oxygenase-1 attenuates brain damage after cerebral ischemia via simultaneous inhibition of superoxide production and preservation of NO bioavailability [J]. Exp Neurol，2013，239（1）：163-169.

[12] Yan Y，Min Y，Min H，et al. n-Butanol soluble fraction of the water extract of Chinese toon fruit ameliorated focal brain ischemic insult in rats via inhibition of oxidative stress and inflammation [J]. J Ethnopharmacol，2014， 151（1）：176-182.

[13] Silva JP，Gomes AC，Coutinho OP. Oxidative DNA damage protection and repair by polyphenolic compounds in PC12 cells [J]. Eur J Pharmacol，2008，601（1/2/3）：50-60.

[14] Shi GF，Wang GY，Chen XF. Screening of radical-scavenging natural neuroprotective antioxidants from Swertia chirayita [J]. Acta Biol Hung，2013，64（3）：267-278.

[15] Patockova J，Marhol P，Tumova E，et al. Oxidative stress in the brain tissue of laboratory mice with acute post insulin hypoglycemia [J]. Physiol Res，2003，52（1）：131-135.

[16] 徐芳琴，郭超，卞，等.丹红注射液对大鼠脑缺血/再灌注损伤治疗时间窗的影响[J].中成药，2016，38（9）：1887-1892.

[17] 葛建彬，卢红建，宋新建，等.枸杞多糖对小鼠脑缺血再灌注损伤的保护作用及其抗氧化应激的机制研究[J].中风与神经疾病杂志，2016，33（9）：790-794.

[18] 方晓艳，郑艳，贾佼佼，等.脑缺血预处理对脑缺血再灌注损伤大鼠抗氧化酶的影响[J].中药药理与临床，2016， 32（3）：193-196.

[19] 陈美华，张燕，陈伟，等.秦皮素对脑缺血再灌注损伤小鼠的保护作用[J].中国老年学杂志，2016，36（10）：2350-2352.

[20] Li Z，Hua C，Pan X，et al. Carvacrol exerts neuroprotective effects via suppression of the inflammatory response in middle cerebral artery occlusion rats [J]. Inflammation，2016，39（4）：1566-1572.

（收稿日期：2016-08-19 本文编辑：张瑜杰）