祛风散寒除湿中药正清风痛宁缓释片对小鼠软骨细胞TGF―β、IL―1β、Fstl―1、TIMP―3表达水平的影响
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【摘 要】目的：探讨祛风散寒除湿法治疗骨关节炎的分子机制。方法：以正清风痛宁缓释片为研究载体，通过实时定量PCR试验，检测软骨细胞转化生长因子-β、白细胞介素-1β、卵泡抑素样蛋白-1、基质金属蛋白酶组织抑制因子-3 mRNA表达水平。结果：在正清风痛宁缓释片水提液高、中、低剂量组（以下简称S100、S50、S25组）以及模型对照组软骨细胞都有软骨细胞转化生长因子-β、白细胞介素-1β、卵泡抑素样蛋白-1、基质金属蛋白酶组织抑制因子-3 mRNA表达。软骨细胞转化生长因子-β mRNA表达量，S100、S50、S25组明显高于模型对照组（P < 0.01），而S25组与模型对照组比较，差异无统计学意义（P > 0.05）；白细胞介素-1β mRNA表达量S50组明显低于模型对照组，差异有统计学意义
（P < 0.01），S100组与模型对照组比较，差异有统计学意义（P < 0.05）；S25组白细胞介素-1β表达量与模型对照组比较，差异无统计学意义（P > 0.05）；卵泡抑素样蛋白-1 mRNA表达量S100、S50、S25组均高于模型对照组，但差异无统计学意义（P > 0.05）；基质金属蛋白酶组织抑制因子-3 mRNA表达量，S50、S100组明显高于模型对照组 （P < 0.01），而S25组与模型对照组比较，差异无统计学意义（P > 0.05）。结论：祛风散寒除湿中药正清风痛宁缓释片对关节软骨具有保护作用，其对骨关节炎软骨的保护作用可能是通过上调软骨细胞转化生长因子-β、卵泡抑素样蛋白-1及基质金属蛋白酶组织抑制因子-3 mRNA在软骨细胞的表达及下调炎性致病因子白细胞介素-1β的表达来实现的。
【关键词】 骨关节炎；祛风散寒除湿法；tgf-β；il-1β；fstl-1；timp-3；正清风痛宁缓释片；软骨细胞；小鼠
doi：10.3969/j.issn.2095-4174.2014.01.004
Effects of Expelling Wind，Removing Cold and Eliminating Dampness TCM Zhengqing Fengtongning Sustained-release Tablets on the Expressions of TGF-β，IL-1β，Fstl-1 and TIMP-3 in Mouse Chondrocytes
WU Jing-jin，PENG Jiang-yun
【ABSTRACT】 Objective：To investigate the molecular mechanism of the method of expelling wind，removing cold and eliminating dampness in the treatment of osteoarthritis.Methods：Zhengqing Fengtongning sustained-release tablets as the carrier was used，by real-time quantitative PCR assay，to detect the mRNA expression levels of TGF-β，IL-1β，Fstl-1 and TIMP-3.Results：There were mRNA expressions of TGF-β，IL-1β，Fstl-1 and TIMP-3 in the chondrocytes of the Zhengqing Fengtongning sustained-release tablets as high，medium and low dose groups （hereinafter referred to as S100，S50，S25 group）and the model control group.The mRNA expressions of TGF-β in the S100，S50，S25 groups were significantly higher than that of the model control group （P < 0.01）.
While compared mRNA expression of TGF-β in the S25 group with that of the model control group，the difference is not significant （P > 0.05）；the mRNA expression of IL-1β in the S50 group was significantly lower than that in the model control group，the difference had statistical significanc（P < 0.01），comparing S100 group with the model control group，the difference was statistically significant （P < 0.05），Comparing IL-1β of the S25 group with that of the model control group，there was no significant difference （P > 0.05）；the mRNA expressions of Fstl-1 of the S25，middle and high dose groups were higher than that of the model control group，but the difference was not statistically significant