Endothelial cell injury with inflammatory cytokine and coagulation in patients w

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BACKGROUND： Current studies on CD62P have focused mainly on cardiovascular diseases， while only few studies have evaluated the effects of CD62P on the development of sepsis and the association between endothelial cell injury with inflammation and coagulation. This study attended to explore the association between endothelial cell injury with inflammation and coagulation by evaluating the expression of soluble CD62P （s-CD62P） in plasma and its mechanism in patients with sepsis， thus to provide the evidence of effective treatment of sepsis with anti-adhesion therapy targeted CD62P.

METHODS： A total of 70 critically ill patients with systemic inflammatory response syndrome （SIRS） admitted to intensive care unit （ICU） between September 2009 and February 2010 were enrolled for a prospective and control study. According to the diagnostic criteria of sepsis/SIRS， the patients were divided into two groups： a sepsis group （n=38） and a SIRS group （n=32）. Another 20 healthy volunteers served as a control group. Patients in the sepsis group and SIRS group were matched by clinical signs of high blood pressure， diabetes and its complications. The demographics of the patients including age， sex， body mass index （BMI）， smoking and alcohol addict were compared among the groups. Six mL peripheral blood samples were collected within 24-hour admission in ICU for enzyme-linked immunosorbent assay （ELISA） to detect the plasma levels of s-CD62P， TNF-α， and hs-CRP. And variables of coagulation function such as platelet （PLT）， prothrombin （PT）， activated partial thromboplastin time （APTT）， D-dimer and antithrombin-III （AT-III） were analyzed during 24 hours after admission to ICU. Meanwhile sequential organ failure assessment （SOFA） score of critically ill patients was evaluated. Data were expressed as mean±standard deviation and were statistically analyzed by using SPSS 17.0 statistical software. The differences in plasma levels of s-CD62P of patients in each group were analyzed by ANOVA and the Kruskal-Wallis test. The relations between s-CD62P and inflammatory cytokines as well as with coagulation were determined by Pearson's product moment correlation coefficient analysis. Changes were considered as statistically significant if P value was less than 0.05.

RESULTS： Compared with the control group and SIRS group， the sepsis group demonstrated significantly higher levels of s-CD62P， TNF-α and highly sensitive C-reactive protein （hs-CRP） （P

CONCLUSIONS： The concentration of plasma s-CD62P is elevated as a early biomarker in patients with sepsis， and it serves as one of the pathogenic factors responsible for endothelial cell damage. Coagulation and mediators of inflammation promote each other， aggravating the severity of sepsis. Plasma s-CD62P may be an important factor for the development of coagulation and inflammatory reaction.

KEY WORDS： Sepsis； Endothelial cell injury； Plasma soluble CD62P； Inflammatory cytokine； Coagulation

World J Emerg Med 2013；4（4）：285289

DOI： 10.5847/ wjem.j.19208642.2013.04.008

INTRODUCTION

Currently， sepsis is considered as a disease process involving progressive endothelial cell injury resulting from disorders of systemic inflammatory and anti-inflammatory balance， activation of leukocyte and platelet， damage of the coagulation system， which ultimately induces multiple organ failure.[1] Granule membrane protein （GMP） CD62P， as an important member of the selectin family of adhesion molecules， is a marker of endothelial cell injury and platelet activation，[2] as well as an important component for triggering and maintaining inflammatory responses. Current studies on CD62P have mainly focused on cardiovascular diseases， whereas few studies have evaluated the effects of CD62P on the development of sepsis and the association between endothelial cell injury with inflammation and coagulation. This study aimed to explore the association between endothelial cell injury with inflammation and coagulation by evaluating the expression of soluble CD62P （s-CD62P） in plasma and its mechanism in patients with sepsis， thus providing the evidence of effective treatment of sepsis with anti-adhesion therapy targeted CD62P.

METHODS

Patients

Seventy patients with systemic inflammatory response syndrome （SIRS）， aged 49.1±19.1 years， 54 males and 16 females， admitted to the intensive care unit （ICU） of the General Hospital of Ningxia Medical University from September 2009 to February 2010， were included in this study. Thirty eight patients were diagnosed with sepsis （sepsis group）， with primary diseases of trauma in 19 patients， pulmonary infection in 5 patients， and abdominal surgery complicated with critical illness in 12 patients. Among the 32 SIRS patients （SIRS group）， primary disease contained trauma in 17 patients， abdominal surgery complicated with critical illness in 10 patients， and selective surgery in 5 patients. Another 20 healthy subjects who underwent physical examination in our hospital served as the control group.

The percentages of patients with hypertension， diabetes and complications in the sepsis and SIRS groups were similar （P>0.05）. Also， there were no significant differences in sex， age， smoking， drinking， and clinical characteristics including body mass index （BMI） （P>0.05 for all） between the three groups （Table 1）.

Patients with chronic diseases or dysfunction of liver （cirrhosis， portal hypertension）， kidney （long-term dialysis） or patients with immune suppression due to immunosuppressive therapy， radio-chemotherapy， leukemia， lymphoma， and acquired immunodeficiency syndrome （AIDS） were excluded from the study. Moreover， patients allergic to anticoagulant drugs， patients with malignancy， peripartum patients， and patients younger than 14 years old were all excluded from the study.

This study was approved by the Ethics Committee of the General Hospital of Ningxia Medical University and informed consents were obtained from all subjects before enrollment.

Laboratory examination

Clinical data of the patients were collected to assess the Sequential Organ Failure Assessment （SOFA） score. Six mL fasting venous blood was collected from the patients in the morning within 24 hours after admission to the ICU and was injected equally into two negative pressure vacuum anticoagulant tubes containing 0.109 mmol/L sodium citrate to obtain a mixture of natrium citricum/whole blood at a ratio of 1：9. The tubes were centrifuged for 10 minutes at 3 000 r/min after mixing. The separated plasma obtained from tube 1 was stored at 80 ?C and ready for examination in batch to measure the levels of s-CD62P and TNF-α by double-sandwich ABC enzyme-linked immunosorbent assay （ELISA） with kits （U.S. R&D company） according to the instructions. The separated plasma obtained from tube 2 was immediately used to measure the coagulation markers which could be stored at room temperature for no more than 2 hours or at 28 ?C for no more than 4 hours.

Statistical analysis

Quantitative data were presented as mean±SD. Non-normal distribution data were presented as median. Student's t test was used for comparison of two groups， variance analysis for comparison of multiple groups， nonparametric rank-sum test （i.e. the Kruskal-Wallis test） for non-paired data with heterogeneous variance， Pearson's product-moment correlation coefficient test for data from normal distribution to assess the relationship. Two-tailed P

RESULTS

Plasma level of s-CD62P

The s-CD62P levels of patients in the sepsis group were significantly higher （P0.05） （Table 2）.

Plasma levels of hs-CRP， TNF-α and SOFA score

The levels of hs-CRP and TNF-α in patients of the sepsis group were significantly higher （P

Count of platelet， PT， APTT， level of D-dimer and activity of AT-III

The patients in the sepsis group and SIRS group showed a higher level of D-dimer and longer PT and APTT （P

Relationship of inflammatory responses with coagulation function/markers

The levels of hs-CRP and TNF-α were positively correlated with D-dimer level， PT， and APTT in patients with critical illness respectively， and were negatively correlated with platelet count and AT-III activity （Table 4）.

Relationship of plasma level of s-CD62P with inflammatory factor and coagulate markers

There was a significant positive relationship between the plasma level of s-CD62P and the levels of hs-CRP and TNF-α in patients with critical illness （P

DISCUSSION

An increasing amount of evidence has shown that endothelial cell injury and activation of platelets induced by the interactions of leukocyte with endothelial cells or platelets play an important role in sepsis development. Activated endothelial cells or platelets induced by tissue hypoxia and/or stimulation of inflammatory mediators first expressed GMP CD62P， mainly found in WeibelPalade bodies of endothelial cells and α-granules of resting platelet， which can mediate initial endothelial/platelet-leukocyte adhesion by binding with P-selectin glycoprotein ligand-1 （PSGL-1） on the surface of these cells and also involve in adhesion and immigration cascades of leukocyte on the surface of the endothelium.[3，4] Then， endothelial cell injury may be induced by secretion of myeloperoxidase， cathepsin G， elastase， and generation of reactive oxygen species of neutrophils after adhesion，[5，6] which could be indirectly evaluated by measuring the levels of these indicators or their metabolic products in the laboratory.[7]

The results of this study demonstrated that the plasma level of s-CD62P of the sepsis group was significantly higher than that of the SIRS group and normal control group. The s-CD62P levels of patients in the SIRS group increased with the severity of SIRS compared with those of patients in the normal control group as shown by mean values， although there was no significant difference between them. This indicated that CD62P was involved in inflammatory response and mediated the development of sepsis， and could be considered as an early biomarker of endothelial cell injury. It has been shown that endothelial cell injury characterized by significantly increased adhesion molecule of α-granule membrane protein 140 （i.e. CD62P） occurred simultaneously with the development of coagulation/fibrinolysis disorders in patients with critical illness.[8] Clinically， endothelial cell injury can be detected by combined measurement of several biomarkers of endothelial injury including circulating endothelial cells （cECs）， endothelial cell specific molecule-1 （ESM-1）， adhesion molecule of CD62P and E-selectin， soluble thrombomodulin （sTM）， angiopoietin-2 （Ang-2）.[9] However， it is difficult to distinguish endothelial cell injury caused by stimulating endothelial cells from vascular endothelial dysfunction caused with detection of these biomarkers， so the application of endothelium-derived active substances measurement in clinical practice has certain limitations.

This study showed that patients in the sepsis and SIRS groups had significantly increased D-dimer level， and prolonged PT and APTT， but significantly decreased AT-III activity compared to the normal control group. Levi et al[10] also confirmed a decreased AT level during severe inflammatory reactions. These results indicated that inflammation would induce the activation of the coagulation system as well as down regulation of physiological anticoagulant mechanisms and inhibition of the fibrinolytic system with the development of sepsis.[11] In addition， our data showed that D-dimer， AT-III， PT and APTT were all significantly related with TNF-α and hs-CRP in patients with critical illness. Plessier et al[12] reported the severity of coagulation dysfunction was closely related to the severity of sepsis in patients with infection. These results further confirmed the close relationship between coagulation system and inflammatory response， the interaction of inflammation and coagulation/fibrinolysis， which will promote the development of sepsis in combination.[13]

The results of our study indicated that CD62P is not only involved in inflammatory response by its relationship with hs-CRP and TNF-α， but also involved in platelet activation and subsequent widespread microthrombosis in microvessels via its relationship with D-dimer， AT-III activity， PT and APTT and that CD62P is an important initiating factor for inflammatory response and coagulation/fibrinolysis dysfunction in sepsis. Mosad et al[14] found in 176 sepsis patients complicated with DIC， CD62P level was positively correlated with the level of DIC， fibrinogen consumption， D-dimer， and markers of thrombin activation. CD62P can not only initiate the inflammatory cascade but also trigger the coagulation cascade. CD62P may initiate the coagulation process by mediating the adhesion of platelets with endothelial cells and leukocytes， which subsequently activate nuclear factor κB （NF-κB） and increase the expression and release of tissue factor （TF） of monocytes， and by subsequent fusion with the membrane of platelets[15] and induce a hypercoagulable state by activating the extrinsic coagulation pathway to produce fibrin.[16] Activated leukocytes can accelerate thrombosis by releasing cathepsin and elastase， hydrolyzing tissue factor pathway inhibitor （TFPI）， and further activating platelets and coagulation factor V， thus releasing reactive oxygen species and leading to direct or indirect activation or damage of vascular endothelium.[16] Currently， the role of CD62P in inflammatory response/coagulation and organ dysfunction in sepsis has been a research topic. Animal experiments found that endothelial injury could increase the expression of adhesion molecules including CD62P， resulting in widespread microvascular inflammation and thrombosis as well as increased mortality of sepsis.[2]

In conclusion， endothelial injury， inflammatory response， and coagulation dysfunction are involved in the whole process of sepsis development， and have interaction with each other triggered by a variety of factors. CD62P and its ligand play an important role in the development of sepsis and organ dysfunction. In an animal study， therapies against CD62P or blocking PSGL-1 were demonstrated to be able to improve organ function and prognosis； simvastatin could reduce the leukocyte aggregation and endotoxemia-induced liver damage by indirect decrease of P-selectin expression.[17] Further studies are needed on the relationship between sepsis， CD62P and clinical treatment， and anti-adhesion therapy targeted on CD62P will provide a new approach to prevent and treat sepsis and complicated organ dysfunction.

Funding： None.

Ethical approval： This study was approved by the Ethics Committee of the General Hospital of Ningxia Medical University； informed consents were obtained from all subjects before enrollment.

Conflicts of interest： None.

Contributors： Ding H proposed the study， analyzed the data and wrote the first draft. All authors contributed to the design and interpretation of the study and to further drafts.

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Received May 6， 2013

Accepted after revision October 11， 2013