载银纳米二氧化钛树脂基托对变异链球菌和白色假丝酵母菌抗菌性的体外研究

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[摘要] 目的 采用体外法研究载银纳米二氧化钛（Ag-TiO2）树脂基托对口腔常见条件致病菌的单一游离菌体及菌斑生物膜的抗菌性能。方法 将不同质量分数的Ag-TiO2抗菌粉剂添加到树脂基托材料中，制成抗菌树脂基托。采用薄膜密贴法分别检测该抗菌树脂基托对变异链球菌、白色假丝酵母菌、金黄色葡萄球菌的抗菌率，并用扫描电镜观察抗菌树脂基托表面变异链球菌、白色假丝酵母菌的生长情况。结果 与未添加抗菌剂相比，抗菌剂质量分数为0.7%时，抗菌树脂基托对金黄色葡萄球菌的抗菌率达93.3%；抗菌剂质量分数为1.5%时，抗菌树脂基托对变异链球菌的抗菌率达90.2%；抗菌剂质量分数为2.5%时，抗菌树脂基托对白色假丝酵母菌的抗菌率也达91.2%。与未添加抗菌剂相比，抗菌树脂基托表面变异链球菌、白色假丝酵母菌的黏附数量明显减少。结论 Ag-TiO2可以显著提高树脂基托的抗菌性能。

[关键词] 载银纳米二氧化钛树脂基托； 抗菌性能； 菌斑

[中图分类号] R 783.6 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.02.022

Study of antibacterial effect of polymethyl methacrylate resin base containing Ag-TiO2 against Streptococcus mutans and Saccharomyces albicans in vitro Liu Jie1, Ge Yali1, Xu Lianli2. （1. Dept. of Prosthodontics, School of Stomatology, Jiamusi University, Jiamusi 154002, China; 2. Dept. of Denture Production, School of Stomatology, Jiamusi University, Jiamusi 154002, China）

[Abstract] Objective To study the antibacterial effect of polymethyl methyacrylate（PMMA） resin base containing

Ag-TiO2 antibacterial agent against common conditioned pathogen and their bacterial plaque in vitro. Methods Diffe-rent qualities of Ag-TiO2 antibacterial agent were added into PMMA resin base in order to prepare antibacterial PMMA resin base. Then, in vitro analysis of antibacterial effect of the resin base against Streptococcus mutans, Staphylococcus aureus, Saccharomyces albicans were assayed with the pellicle-sticking method. Furthermore, the growth of Streptococ-cus mutans and Saccharomyces albicans bacterial plaque on the antibacterial PMMA resin base were examined by scanning electron microscope. Results The inhibition percent for Staphylococcus aureus was 93.3% by antibacterial PMMA resin base with 0.7% Ag-TiO2; for Streptococcus mutans 90.2% with 1.5% Ag-TiO2; for Saccharomyces albi-cans 91.2% with 2.5% Ag-TiO2. Bacterial plaque of Streptococcus mutans and Saccharomyces albicans on antibacterial resin base were inhibited effectively. Conclusion The antibacterial property of the PMMA resin base is effectively enhanced with Ag-TiO2 antibacterial agent.

[Key words] polymethyl methacrylate resin base containing Ag-TiO2； antibacterial effect； bacterial plaque

随着全球逐渐步入老龄化社会，佩戴活动义齿或全口义齿的患者越来越多。义齿的戴入常造成口腔原有微生态环境改变，导致口腔条件致病菌增多；聚甲基丙烯酸甲酯制成的人工牙及树脂基托由于其独特的理化性能，易黏附细菌、真菌且难以彻底清除，从而继发一系列口腔疾病[1]。因此，如何提高义齿的抗菌性能是目前国内外关注的热点之一。

近年来，无机抗菌剂凭借其抗菌谱广、抗菌时效长、耐高温、生物安全性好等特性得到国内外学者一致认可。无机抗菌剂中，一类是通过物理吸附、离子交换等方法，将银、铜、锌等抗菌金属离子负载在沸石、硅胶等多孔材料表面制成的抗菌剂；另一类是利用材料自身光催化作用产生大量含氧自由基（reactive oxygen species，ROS）发挥杀菌作用的钛类（TiO2）抗菌剂[2]。本研究将载银纳米二氧化钛（Ag-TiO2）抗菌剂添加到树脂基托中，采用体外实验，探