牙周炎对动脉粥样硬化影响的动物实验研究

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[摘要] 目的 建立牙周炎和动脉粥样硬化（AS）动物复合模型，探讨牙周炎对AS的影响。方法 36只日本大耳白兔随机分为4组，包括单纯牙周炎（CP）组、牙周炎合并AS（CP+AS）组、单纯AS组和对照组，采用结扎结合涂牙龈卟啉单胞菌（P. gingivalis）的方法建立牙周炎动物模型，单侧髂动脉球囊内皮损伤术建立AS模型。建模成功后，采用苏木精-伊红（HE）染色观察样本的组织病理学改变；Elastica van Gieson（EVG）弹力纤维染色进行形态分析，计算髂动脉横断面内膜与中膜面积的比值。采用巢氏聚合酶链反应（PCR）法检测受损动脉壁中P. gingivalis 16S rDNA。酶联免疫吸附测定（ELISA）法检测系统炎性因子的表达，包括C反应蛋白（CRP）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）。结果 CP+AS组的CRP、IL-6及IL-1β表达水平较其他组明显升高（P

[关键词] 牙周炎； 动脉粥样硬化； C反应蛋白； 白细胞介素-6； 白细胞介素-1β

[中图分类号] R 781.4 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.03.021

An animal experiment study on the effect of periodontitis on atherosclerosis Wang Zhou1, Zhang Mingzhu1, Yu Zhuo2, Shui Yanqing1, Ding Yun1, Lei Yayan1. （1. Dept. of Oral Medicine, The Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650031, China; 2. Dept. of Cardiovascular Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming 650031, China）

[Abstract] Objective To make an animal periodontitis and atherosclerosis compound model, and to study the effects of periodontitis on atherosclerosis. Methods 36 Japan rabbits were randomly divided into four groups: Including perio-dontitis model group, periodontitis and atherosclerosis compound model group, atherosclerosis model group and control group. Periodontitis model was initiated by ligating floss around teeth cervical and oral inoculating with Porphyromonas gingivalis（P. gingivalis）. Atherosclerosis was established by single iliac artery of balloon-injured rabbit. Histopathological change of injured iliac artery was observed under optical microscope after hematoxylin-eosion stain. Elastica van Gieson-

stained sections were used for the morphometric analysis. We measured intimal and medial lesion areas in iliac artery cross-sections as well as the intimal/medial ratio（I/M）. We also analyzed P. gingivalis 16S rDNA amplification with

nested-polymerase chain reaction（nested-PCR）, and detect systemic proinflammatory mediators with enzyme linked im-

munosorbent assay（ELISA）, including C-reactive protein（CRP）, interleukin-6（IL-6） and interleukin-1β（IL-1β）. Results The serum levels of CRP, IL-6 and IL-1β increased obviously among the compound model group than other groups（P