惠州客家人群食管癌EGFR蛋白表达及与MAPK信号通路相关性的初步研究

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[摘要] 目的 检测EGFR在惠州客家人群食管癌组织的表达，探讨egfr与惠州客家人群食管癌发生发展之间的关系及与MAPK信号传导激活状态的相关性。 方法 采用免疫组织化学及免疫蛋白印迹方法，检测53例惠州客家人群食管癌和癌旁正常组织标本中EGFR、MAPK（ERK1/2）和p-ERK1/2蛋白的表达。 结果 EGFR和p-ERK1/2在食管癌组织中的表达率显著高于癌旁正常组织（P < 0.01）；分化程度越低，EGFR和p-ERK1/2的表达越高；有淋巴结转移患者EGFR和p-ERK1/2的表达明显高于无淋巴转移者（P < 0.01）；浸润浅肌层患者的p-ERK1/2表达阳性率明显低于浸润深肌层（P < 0.05）。 结论 EGFR/mapk信号传导与惠州客家人群食管癌的发生发展密切相关。伴随EGFR的过表达，MAPK（ERK1/2）信号通路活化水平升高，EGFR/MAPK信号通路可为食管癌的早期基因治疗提供新的靶点。

[关键词] 食管癌；惠州客家人群；EGFR蛋白；MAPK蛋白；信号传导

[中图分类号] R735.1 [文献标识码] A [文章编号] 1673-7210（2012）08（c）-0016-04

Preliminary study in Huizhou Hakka population of esophageal cancer EGFR protein expression and correlation with MAPK signaling pathway

PEI Xiaojuan1 YANG Qingxu1 ZOU Dongmei2 ZHU Yingling1

1.Department of Pathology, Central People's Hospital of Huizhou City, Guangdong Province, Huizhou 516001, China；2.Chinese Medical Association Huizhou Dongjiang Pathology Diagnosis Center, Guangdong Province, Huizhou 516001, China

[Abstract] Objective To investigate the expression of EGFR protein in esophageal cancer of Huizhou Hakka population, discussing the relationship EGFR and MAPK activation of signal transduction in the occurrence and development of esophageal cancer of Huizhou Hakka population. Methods Immunohistochemical staining and Western blot was employed to detect the expression of EGFR, MAPK and p-ERK1/2 protein expression in 53 cases of esophageal cancer and normal esophageal tissues adjacent to cancer. Results EGFR and p-ERK1/2 expression in esophageal cancer were significantly higher than in normal esophageal tissues adjacent to cancer (P < 0.01); the lower the degree of differentiation, the higher expression of EGFR and p-ERK1/2; expression of EGFR and p-ERK1/2 with lymph node metastasis patients were higher than who without lymph node metastasis (P < 0.01); positive expression rate of p-ERK1/2 with the superficial myometrial infiltration patients was remarkablely lower than that of depth of myometrial infiltration (P < 0.05). Conclusion EGFR/MAPK signal transduction is closely related to the occurrence and development of esophageal cancer in Huizhou Hakka population. Accompanied by EGFR over expression, elevating the level of activated MAPK (ERK1/2) signaling pathway, so EGFR /ERK proteins may to provide a new target for early gene therapy in Huizhou Hakka population esophageal cancer.

[Key words] Esophageal Carcinoma; Huizhou Hakka population; EGFR protein; MAPK protein; Signal transducation